RESUMO
The behavior of magnetite nanoparticles was studied in the cell chip microcapillaries. No aggregation of magnetite nanoparticles under conditions of long-term circulation was noted. Biodistribution and toxicity of magnetite nanoparticles (14 nm) and aminated magnetite after their intragastric administration to mice were studied in vivo. According to mass spectrometry and microscopy data, accumulation of nanoparticles occurred mainly in the liver cells.
Assuntos
Nanopartículas de Magnetita/química , Animais , Animais não Endogâmicos , Avaliação Pré-Clínica de Medicamentos , Estabilidade de Medicamentos , Fígado/efeitos dos fármacos , Fígado/metabolismo , Fígado/patologia , Nanopartículas de Magnetita/toxicidade , Masculino , Camundongos , Microcirculação , Técnicas Analíticas Microfluídicas , Modelos Biológicos , Soluções , Distribuição TecidualAssuntos
Resistencia a Medicamentos Antineoplásicos/efeitos dos fármacos , Resistencia a Medicamentos Antineoplásicos/genética , Expressão Gênica/efeitos dos fármacos , Neoplasias Pulmonares/tratamento farmacológico , Procedimentos Analíticos em Microchip/métodos , Paclitaxel/farmacologia , Antineoplásicos Fitogênicos/farmacologia , Linhagem Celular Tumoral , Humanos , Concentração Inibidora 50RESUMO
The incidence of SLC6A4 gene 5HTTLPR polymorphism alleles was evaluated in 223 male athletes engaged in endurance sports, the results were compared with those in 177 male nonathletes. Association between 5-HTTLPR genotypes and the effect of exhaustive treadmill running on simple and complex visual reactions and critical flicker frequency threshold was studied. We found that the incidence of LL genotype was significantly higher in athletes in comparison to nonathletes; after exercise, the velocity of visual reactions and critical flicker frequency increased; exercise did not change the velocity of complex visual reaction in LL-carriers, and increased it in SS-carriers. We conclude that exhausting treadmill running leads to facilitation sensory information processing in athletes and that SS-carriers are more susceptible to the effect of exhaustive treadmill running than LL-carriers.
Assuntos
Atletas , Exercício Físico/fisiologia , Tempo de Reação/genética , Proteínas da Membrana Plasmática de Transporte de Serotonina/genética , Adulto , Genótipo , Humanos , Masculino , Resistência Física/genética , Polimorfismo de Nucleotídeo Único , Adulto JovemRESUMO
Changes in nerve conduction after 10-min electroneurostimulation of the posterior surface of the neck were studied. Changes in the parameters of laser-induced potentials obtained during stimulation of C7 dermatome on hands and posterior surface of the neck were found. Decrease in the amplitude and shortening of the component latency were shown. Method of laser-induced potentials was concluded to provide unbiased estimation of the level and peculiarities of analgesic effects of physical factors.
Assuntos
Potenciais Somatossensoriais Evocados/fisiologia , Condução Nervosa/fisiologia , Estimulação Elétrica Nervosa Transcutânea/métodos , Adulto , Mãos/fisiologia , Humanos , Terapia a Laser , Masculino , Pessoa de Meia-Idade , Pescoço/fisiologia , Nociceptores/fisiologiaRESUMO
Study by means of expression profiling with Human GeneChip ST1.0 microchips (Affymetrix) revealed 10 early immune response genes, whose expression was modified after short-term intense physical exercise. They include genes for immunoglobulin-like receptors of natural killer cell, genes for IL-2Rß and IL-18RAP receptors, and two genes for functional proteins (perforin 1 and granzyme B) that provide the cytotoxic potential of killer cells. Possible mechanisms of stimulation, activation of the receptor apparatus, and cytotoxic effect on natural killer cells are evaluated under conditions of physical exercise.
Assuntos
Exercício Físico/fisiologia , Regulação da Expressão Gênica/fisiologia , Receptores de Células Matadoras Naturais/metabolismo , Regulação da Expressão Gênica/imunologia , Granzimas/metabolismo , Humanos , Subunidade beta de Receptor de Interleucina-2/metabolismo , Masculino , Análise de Sequência com Séries de Oligonucleotídeos , Perforina , Proteínas Citotóxicas Formadoras de Poros/metabolismo , Receptores de Interleucina-18/metabolismo , Receptores de Células Matadoras Naturais/genética , Adulto JovemAssuntos
Antineoplásicos/farmacologia , Doxorrubicina/farmacologia , Resistencia a Medicamentos Antineoplásicos/genética , Perfilação da Expressão Gênica , Neoplasias Pulmonares/genética , Neoplasias Pulmonares/patologia , Biomarcadores Tumorais/genética , Linhagem Celular Tumoral , Regulação Neoplásica da Expressão Gênica/efeitos dos fármacos , Regulação Neoplásica da Expressão Gênica/genética , Humanos , Transdução de SinaisRESUMO
Intensive exercise triggers the cascade processes of body adaptation, including modulation of splisosome functioning, and can lead to modification of its activity and choice of alternative exons. We studied the effect of exercise of the maximum aerobic power on activation of transcription of genes involved in the splicing process. Short-term exercise resulted in a significant increase of mRNA expression of genes encoding proteins involved in the formation of precatalytic splisosome: DDX17, DDX46, HNRNPR, PRPF4B, and SRPK2. The role of the detected regulators in initiation of splisosome assembly under conditions of maximally intensive exercise is discussed.
Assuntos
Exercício Físico/fisiologia , Regulação da Expressão Gênica , Splicing de RNA/genética , Adolescente , RNA Helicases DEAD-box/genética , RNA Helicases DEAD-box/fisiologia , Perfilação da Expressão Gênica , Ribonucleoproteínas Nucleares Heterogêneas/genética , Ribonucleoproteínas Nucleares Heterogêneas/fisiologia , Humanos , Análise de Sequência com Séries de Oligonucleotídeos , Proteínas Serina-Treonina Quinases/genética , Proteínas Serina-Treonina Quinases/fisiologia , Ribonucleoproteína Nuclear Pequena U2/genética , Ribonucleoproteína Nuclear Pequena U2/fisiologia , Ribonucleoproteína Nuclear Pequena U4-U6/genética , Ribonucleoproteína Nuclear Pequena U4-U6/fisiologia , Adulto JovemRESUMO
Muscular dystrophy is a common multisystem disease, which results from the impairment of alternative splicing. An increase in the number of unstable CTG and CCTG repeats in untranslated regions of the DMPK and ZNF9 genes is followed by dysregulation of RNA-binding proteins. Further changes are followed by dysfunction of insulin receptors, membrane Cl- channels, and other proteins. We developed a new mathematical model for the regulation of splicing of exon 11 in the IR gene, which describes the effect of various factors on alternative splicing.
Assuntos
Processamento Alternativo/genética , Modelos Genéticos , Distrofia Miotônica/genética , Receptor de Insulina/genética , Humanos , Modelos BiológicosRESUMO
The method of mid-infrared molecular spectroscopy allows precise measuring of the concentrations of a large number of biological molecules in a minimal sample volume. Method of projections on latent structures was used for plotting the calibration models. On the basis on mid-infrared spectral data we obtained calibration models for calculation of serum content of various substances: albumin, cholesterol, glucose, total protein, urea, 70 Da heat shock protein, and malonic dialdehyde.
Assuntos
Análise Química do Sangue/métodos , Soro/química , Espectrofotometria Infravermelho/métodos , HumanosRESUMO
Associations of functional polymorphism in genes of dopamine receptor DRD2, dopamine transporter DAT, and dopamine degrading enzyme COMT with variations in anxiety sensitivity threshold were studied. On the basis of genetic and psychological analysis, an attempt was undertaken to evaluate the relationship of DRD2, DAT and COMT genotypes with values obtained using different anxiety scales. It was found that carriers of VA1(+)9(+) genotype exhibit increased anxiety and significantly differed from individuals with other genotypes. The results were illustrated with model of dopamine diffusion in the extracellular space of the striatum in carriers of different genotypes.
